Original Research Article I Volume 5 I Issue 2 I 2019
In Vitro Screening of 20 Indian Medicinal Plants for their Anti-Cholinesterase Effects
D.Sripriya, T. Suvarna
The American Journal of Science and Medical Research; 5(2); 1-5
DOI:http://dx.doi.org/10.17812/ajsmr521
Abstract:
The purpose of the present study is to contribute to an understanding of the actual and potential role of 20 traditional medicinal plants based Methanol extracts role in the Enzyme inhibition Assays against Alzheimer’s disease. The objective of the study was to investigate the Acetyl cholinesterase Enzyme inhibitory activity of 90% methanol extracts of 20 selected medicinal plants. Quantitative Analysis made with the help of 96 wells Micro plate technique. Readings have been done with the help of Micro plate reader. Calculation of IC-50 Values by interpolation of a RIA (or) ELISA interpolates unknowns from sigmoid curve using Graph Pad PRISM2.8.1.1 (330) Software. Comparison of IC50 Values with standard. In the present study, methanol extracts of 20 plants used in Indian Ayurvedic system of medicine for improving cognitive function were screened for acetyl cholinesterase inhibitory activity by Ell man’s micro plate calorimetric method against Alzheimer’s disease.
Keywords:
Alzheimer’s, Graph Pad PRISM, Acetylcholine, Microplate, Medicinal plants
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Article Dates:
Received: 3 March 2019; Accepted: 7 April 2019; Published: 21 May 2019
How To Cite:
http://dx.doi.org/10.17812/ajsmr521 Received :3 March, 2019 Accepted; 7April, 2019 Available online :21 May, 2019
