Original Research Article I Volume 4 I Issue 1 I 2018
Antibacterial Activity of Actinomycetes Collected From an Extreme Environment and Coal Mines
B.V Gopinath and M.A Sinagara Charya
The American Journal Science and Medical Research; 4(1); 1-5
DOI:http://dx.doi.org/10.17812/ajsmr4101
Abstract:
Actinomycetes are saprophytic bacteria that secrete important hydrolytic enzymes, antibiotics and medicinally important secondary metabolites. The objective of this study is to study the antibacterial activity of actinomycetes collected from an extreme environment and coal mines. Soil sample were collected from five important coal field regions of Singareni Colleries Company Limited (SCCL), Telangana State. A total of 106 actinomycetes were isolated by perpendicular streak method against gram-positive test bacteria i.e. Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Staphylococcus aureus, Micrococcus luteus, Bacillus stearothermophilus and gram-negative test bacteria i.e. Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Klebsiella pneumoniae species. The isolated actinomycetes were identified using different staining techniques, various biochemical methods and molecular approaches. Antibacterial compound was recovered from the filtrate grown with different actinomycetes strains by solvent extraction method. Eight actinomycetes strains isolated from coalmine soils were used in the screening and the actinomycetes isolates were cultured on glycerol-broth, starch-casein broth, ISP-2 and ISP-3 at 28OC. The antimicrobial activity was determined by agar well diffusion method. It was observed that two isolates were active against only gram-negative bacteria and sixteen against gram-positive bacteria and twenty six against gram-positive and gram-negative bacteria. Finally sixteen isolates were selected for further study on the basis of a) broad spectrum activity and b) layer zone of inhibition in comparison to others.
Keywords:
Actinomycetes, antibiotics, coal mines, SCCL.
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Article Dates:
Received: 12 December 2017 ; Accepted: 21 January 2018 ; Published: 21 February 2018
How To Cite:
http://dx.doi.org/10.17812/ajsmr4101 Received : 12 December, 2017 Accepted; 21 January, 2018 Available online : 21 February, 2018
