Original Research Article I Volume 3 I Issue 3 I 2017
Evaluation of Antibacterial activity of fruit and root extracts of Withania somnifera against Human pathogenic bacteria
Kadudula Prasuna and Yalavarthy Prameela Devi
The American Journal of Science and Medical Research; 3(3); 12-15
DOI:http://dx.doi.org/10.17812/ajsmr3303
Abstract:
Withania somnifera, also known as ashwagandha, is an important herb in ayurvedic and indigenous medical systems. The present study was designed to evaluate the antibacterial activities of an 80% aqueous methanolic extract of W. somnifera roots and fruits. Antibacterial activities were measured using the agar well diffusion method and MIC values were determined by microdilution method and five pathogenic Gram-negative bacteria: Escherichia coli, Salmonella typhi, Citrobacter freundii, Pseudomonas aeruginosa and Klebsiella pneumoniae. The root extracts displayed the highest activity against S. typhi (32.00 ± 0.56 mm zone of inhibition), whereas the lowest activity was against K. pneumoniae (19.00 ± 1.56 mm zone of inhibition). The lowest minimum inhibitory concentration value was 6.25 mg/ml, which was against S. typhi, followed by 12.5 mg/ml against E. coli. It will be beneficial to investigate the active compounds present in W. somnifera so that its roots can be used to increase the armamentarium of antimicrobial agents and so that other possible therapeutic uses of the plant can be explored.
Keywords:
Antibacterial activity, Withania somnifera, methanol, MIC, agar well diffusion method,
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Article Dates:
Received: 30 May 2017; Accepted: 21 July 2017; Published: 29 July 2017
How To Cite:
Pranavi Sreeramoju and Estari Mamidala (2017). An Ethnobotanical Survey of Antidiabetic plants used by tribes of Warangal District, Telangana State. The Ame J Sci & Med Res, 3(3):8-11. doi:10.17812/ajsmr3302.
