Original Research Article I Volume 2 I Issue 3 I 2016
Immobilization and Characterization of Catalase Enzymes from Potato Extract by Sodium Alginatet
Kranthi Kumar G
American Journal of Science and Medical Research; 2(3); 22-25
DOI:http://dx.doi.org/10.17812/ajsmr324
Abstract:
To control oxidative stress, the eukaryotic cell yields various ROS-scavenging enzymes such as superoxide dismutase (which reduces O2 to H2O2), glutathione peroxidase, and catalase. In this work catalase was moderately purified from potato and immobilized against sodium alginate and its catalytic assets in view of the pH and temperature properties were examined. (NH4)2SO4 precipitation and dialysis were made in the extracted enzyme. The pH and temperature optima of the immobilized and free catalase were examined. Optimum temperature was detected higher than that of the free enzyme. The optimum pH was the same for both free and immobilized catalases (pH 7.50). The immobilized catalase was inhibited by anionic stabilizers and the immobilized enzyme presented higher strengths than the free enzyme.
Keywords:
Catalse, ROS, pH, Temperature, immobilization
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Article Dates:
Received: 2 July 2016; Accepted: 4 August 2016; Published: 28 September 2016
How To Cite:
Kranthi Kumar G (2016). Immobilization and Characterization of Catalase Enzymes from Potato Extract by Sodium Alginatet. The Ame J Sci & Med Res, 3(2): 22-25. doi:10.17812/ajsmr324.
