Original Research Article I Volume 2 I Issue 1 I 2016
Detection of serinecarbapenemase and Metallocarbapenemase enzymes in Klebsiella pneumoniae in a tertiary care hospital
B. Radhika and I. Jyothi Padmaja
American Journal of Science and Medical Research; 2(1); 136-147
DOI: http://dx.doi.org/10.17812/ajsmr2016211
Abstract:
Various carbapenems have been reported in K.pneumoniae such as KPC,VIM,NDM & OXA-48 etc. In addition, carbapenemase producers are usually associated with many other non – β-lactam resistance determinants which give rise to multidrug and pan drug resistant isolates. Detection of these enzymes in infected patients and in carriers are the two main approaches for prevention of their spread. Potential carbapenamase producers are currently screened 1st by susceptibility testing, using breakpoint values for carbapenems. However many carbapenemase producers donot confer obvious resistance levels to carbapenems. So there is need for laboratories to search for carbapenemase producers.
Keywords:
Imipenem, Serine carbapenemase, Metallocarbapenemase,Modified Hodge test, Disc Synergy test.
References:
1. Anne Marie Queenan and Karen Bush Carbapenemase – a versatile β-lactamase Article - Antimicrobial Agents Chemotherapy. Johnson & Johnson Pharmaceutical Reasearch and Development, L.L.C Rantan, NJ 08869.(2001).Pg: 1151-1161.
2. Betty A.Forbes, Daniel F.Sahm, Alice S.Weissfeld Bailey & Scott’s Diagnostic Microbiology 12th edition pg: 218-247
3. Colle J.G, Fraser A.G, Marmion B.P, Simmons A Mackie & Mc Cartney Practical medical Microbiology 14th edition pg:131-149)
4. Carla Fontana, Marco Favaro, Loredana sarmati, Silvia Natoli, Anna Altieri,Maria C Bossa, Silvia Minelli, Francesca Leonardis, Cartesio. Emergence of KPC-producing K.pneumoniae in Italy. Antimicrobial agents and Chemotherapy, August 2007.
5. David Greenwood, Richard C.B.Slack, John F.Peutherer A guide to Microbial Infections: Pathogenesis, Immunity, Laborator Diagnosis and Control. 16th edition, pg: 275-277
6. D.R.Arora Text book of Microbiology 3rd Edition. Pg no-356.
7. Dennis.S.Hansen, Hazel.M.Aucken, Titi Abiola, Rainer Podschun Recommended test panel for differentiation of Klebsiella species on the basis of a trilateral interlaboratory evaluation of 18 biochemical tests. Journal of Clinical Microbiology 2004,42(8):3665-9.
8. E kta Gupta, Srujana Mohanty, Seema Sood, Benu Dhawan, Bimal K. Das and Arti Kapil. Emerging resistance to carbapenems in a tertiary care hospital in North India. Indian journal of medical research, July(2006). Pg: 95-98.
9. Fareya Haider, Vineeta Mittal, M.E.Siddique. Evaluation of three phenotypic methods for detection of Metallo-beta- lactamases in Gram negative bacilli from hospitalized patients. Microbes, Molecules & Beyond, XXXV National conference of Indian.Association of Medical Microbiologists, Abstracts and Souvenir.
10 George A.Jacoby, Debra M.Mills and Nancy Chow Role of β- lactamases and porins in resistance to Ertapenem and other β- lactams in Klebsiella pneumoniae. Antimicrobial Agents Chemotherapy.(2004). Pg: 3203-3206.
Article Dates:
Received: 2 December 2016; Accepted: 16 January 2016; Published: 3 February 2016
How To Cite:
B. Radhika and I. Jyothi Padmaja (2016). Detection of serinecarbapenemase and Metallocarbapenemase enzymes in Klebsiella pneumoniae in a tertiary care hospital. The American Journal of Science and Medical Research, 2(1):136-147. doi:10.17812/ajsmr2016211. Received 2 December, 2016; Accepted 16 January, 2016; Published online 3 February, 2016
