Research Article I Volume 10 I Issue 3 I 2024

Histological alterations in the liver tissue of Channa punctatus on exposure to the drug Diclofenac

P. Rohini

Biolife, Volume 10, Issue 3, 2014, pp 1-7



Pharmaceuticals have emerged as priority pollutants in recent times. Diclofenac is a widely prescribed non steroidal anti-inflammatory drug. It has been frequently detected in the surface waters worldwide. There are many experimental evidences on the toxicity of the drug Diclofenac in the aquatic flora and fauna. The present study aims to investigate the histological alterations caused by Diclofenac in the liver tissue of the fresh water fish, Channa punctatus. The acute toxicity test was conducted by exposing the fish to ten different concentrations of Diclofenac. The median lethal concentration was found to be 25.28mg/L by probit analysis. The fish were then exposed to sub-lethal (8.42mg/L) and median lethal (25.28 mg/L) concentrations of Diclofenac for a period of 96 hours. The histological alterations in liver were studied by adopting standard method. The exposed fish have shown marked pathological changes in the liver tissue when compared to control. The pathological changes noticed include degeneration of cytotoplasm in hepatocytes, severe necrosis, atrophy, vacuolization, rupture in blood vessels, disappearance of hepatocyte wall, disposition of hepatic cords, appearance of blood streaks, syncytial appearance of hepatocytes, increase of sinusoidal space and evacuation of portal systems. The findings of the study have clearly shown the toxic effect of the drug Diclofnac in the non target organisms like fish.


Channa punctatus, Diclofenac, valuolization, lethal, probit analysis.


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Received: 14 May 2024; Accepted: 20 June 2024; Published: 04 July, 2024

How To Cite:

P. Rohini. (2024). Histological alterations in the liver tissue of Channa punctatus on exposure to the drug Diclofenac. In Biolife (Vol. 10, Number 3, pp. 1–7). Zenodo.

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