Original Research Article I Volume 7 I Issue 2 I 2021
Study on acute toxicity and haematological alterations induced by the exposure of ibuprofen to common carp (Cyprinus carpio)
Kurhade Chandrakant Shankarrao and U.M Jayabhaye
The American Journal of Science and Medical Research
DOI:https://dx.doi.org/10.17812/ajsmr.2021.721
Abstract:
Indiscriminate discharge of pharmaceutical waste into the aquatic ecosystem may pose major health challenges to aquatic biota. The effect of acute exposure to ibuprofen was studied utilizing changes in haematological parameters under static bio-assay method in common carp (Cyprinuscarpio). For this investigation, the fishes are split into four separate groups. Based on the sub-lethal toxicity research, fishes exposed to 1/10th of LC50 value of ibuprofen. The 96-h LC50 of Ibuprofen was 12.75 mg/L. in C. carpio. Therefore, for this experiment, fish were subjected to 0.25, 0.50 and 1 mg/L doses of ibuprofen. At the end of each exposure session (7th day, 14th day and 21st day), fish specimens were anesthetised with tricaine methane sulfonate to permit the collection of blood samples. The RBC count in group IV fishes of ibuprofen treated fishes, at 21 day of exposure, was lowered and it is statistically significant (p<0.01) to control fishes. WBC counts in fishes that had 7 and 14 days exposure to ibuprofen were not statistically altered, compared to control rats. Ibuprofen treated fish did not elicit any significant alterations (p ˃ 0.05) with reference to monocytes, basophils and eosinophils. Based on the above results, it might be inferred that administration of ibuprofen, may elicit immunological perturbations, and their toxicity may rise depending on the dose.
Keywords:
Haemotology, C. carpio, Acute toxicity, WBC, RBC
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Article Dates:
Received: 2 April 2021; Accepted: 21 May 2021 ; Published: 4 June 2021
How To Cite:
https://dx.doi.org/10.17812/ajsmr.2021.721 Received: 2 April 2021; Accepted: 21 May 2021 Published: 4 June 2021
