Original Research Article I Volume 8 I Issue 3 I 2022

Inhibition of Human Immunodeficiency Virus (HIV-1) Reverse Transcriptase by Acalypha indica (L.) Plant Leaves Extract

Prasad Paindla, Estari Mamidala

The American Journal of Science and Medical Research (2022), 8(3); 1-4

DOI: https://dx.doi.org/10.5281/zenodo.7213389

Abstract:

HIV (Human Immunodeficiency Virus) the virus that bases AIDS, is one of the newest zones of medical research nowadays. The objective of the study is to test the HIV-1 reverse transcriptase inhibitory efficiency of Acalypha indica leaves extracts. The leaves of Acalypha indica, were collected from the selected places of Adilabad District. Leaves were exposed to size reduction to get coarse powder and extracted by sequential maceration method using non-polar to polar solvents. Cell viability was determined in contradiction of PBMC cells by the trypan-blue dye exclusion technique and by MTT assay. The HIV reverse transcriptase enzyme inhibition due to each extract was determined by HIV- 1 RT inhibition assay by using of Retro Sys HIV-1 RT activity kit. At 50 µg/mL n-hexane extract presented the uppermost percentage of HIV-1 RT enzyme inhibition (88.26%) followed by methanol (75%), chloroform (67.3%), ethyl acetate (67.3%) and acetone (63.4%). The weak inhibition was establish at 3.125 µg/mL concentration for all extracts, except n-hexane. At this concentration n-hexane displays more than 50% inhibition. The control drug AZT shows highest inhibition (88.26% and 92.6%) at 25 and 50 µg/mL concentrations. These results achieves that n-hexane crude extract taking more effective activity among the other crude extracts.

Keywords:

Acalypha india, HIV, MTT assay, reverse transcriptase, trypan blue.

References:

  1. Khare VS Singh VP, Sharma SK. Medicinal plants from Ujjain district Madhya Pradesh - part II. Indian Drugs Pharm Ind 1980; 5: 7-12.
  2. Gupta K. and P.J. Klasse. (2006). How do viral and host factors modulate the sexual transmission of HIV? Can transmission be blocked? PLoS Med, 3(2): 79.
  3.  Kirtikar K.R., Basu B.D., Indian Medicinal Plants, International book distributors, 2006,   (2), 856 -860.  
  4. K. M. Nadkarni. Indian Materia Medica, Bombay Popular Prakashan, 2009, Vol.1, 285-    286
  5. R. N. Chopra, S. L. Nayar, I. C. Chpora. Glossary of Indian Medicinal Plants, NationalInstitute of Science Communication and Information Resources, 2006, page no.54-56.
  6. S. S. Agarwal, M. Paridhavi, Clinically useful herbal drugs, Ahuja Publishing House, 2005, 281-282.
  7. Ben Erik, Van Wyk, Michael Wink, Medicinal Plants of the World, Briza Publications, 2009, page no. 403.  
  8. Anazetti MC, Melo PS, Duran N, Haun M (2003). Comparative cytotoxicity dimethylamide-crotonin in the promyelocytic leukemia cell line (HL60) and human peripheral blood mononuclear cells. Toxicology 188: 261-274
  9. Liu CP, Tsai WJ, Lin YL, Liao JF, Chen CF, Kuo YC (2004). The extracts from Nelumbo nucifera suppress cell cycle progression, cytokine genes expression, and cell proliferation in human peripheral blood mononuclear cells. Life Sci. 75: 699-716.
  10. Estari Mamidala, & Prasad Paindla. (2013). Ethnobotanical Survey In Different Mandals Of Adilabad District, Andhra Pradesh, India. Dr. Estari Mamidala, Ethnobotanical Survey in Different Mandals of Adilabad District, Andhra Pradesh, India, International Journal of Sciences 01(2013):77-83, 2(1), 77–83. https://doi.org/10.5281/zenodo.7213355
  11. Estari Mamidala, & Prasad Paindla. (2014). Phytochemical and Chromatographic Studies in the Leaves Extract of Acalypha Indica. International Interdisciplinary Research Journal, 4(1), 1750182. https://doi.org/10.5281/zenodo.7213379
  12. WHO, (2014).  Report on the global HIV/AIDS, Epidemic.
  13. NACO, (2011).  Annual Report 2010-2011
  14. AVERT, (2011). AIDS Virus Education and Research Trust History of AIDS.

Article Dates:

Received: 12 June 2022; Accepted: 10 August, 2022; Published: 3 September, 2022

How To Cite:

Prasad Paindla, & Estari Mamidala. (2022). Inhibition of Human Immunodeficiency Virus (HIV-1) Reverse Transcriptase by Acalypha indica (L.) Plant Leaves Extract. The American Journal of Science and Medical Research, 8(3), 1–4. https://doi.org/10.5281/zenodo.7213389

Download Full Paper: PDF
Submit Manuscript
Join as Reviewer / Editor

Logo