Original Research Article I Volume 8 I Issue 3 I 2022

Inhibition of Human Immunodeficiency Virus (HIV-1) Reverse Transcriptase by Acalypha indica (L.) Plant Leaves Extract

Prasad Paindla, Estari Mamidala

The American Journal of Science and Medical Research (2022), 8(3); 1-4

DOI: https://dx.doi.org/10.5281/zenodo.7213389


HIV (Human Immunodeficiency Virus) the virus that bases AIDS, is one of the newest zones of medical research nowadays. The objective of the study is to test the HIV-1 reverse transcriptase inhibitory efficiency of Acalypha indica leaves extracts. The leaves of Acalypha indica, were collected from the selected places of Adilabad District. Leaves were exposed to size reduction to get coarse powder and extracted by sequential maceration method using non-polar to polar solvents. Cell viability was determined in contradiction of PBMC cells by the trypan-blue dye exclusion technique and by MTT assay. The HIV reverse transcriptase enzyme inhibition due to each extract was determined by HIV- 1 RT inhibition assay by using of Retro Sys HIV-1 RT activity kit. At 50 µg/mL n-hexane extract presented the uppermost percentage of HIV-1 RT enzyme inhibition (88.26%) followed by methanol (75%), chloroform (67.3%), ethyl acetate (67.3%) and acetone (63.4%). The weak inhibition was establish at 3.125 µg/mL concentration for all extracts, except n-hexane. At this concentration n-hexane displays more than 50% inhibition. The control drug AZT shows highest inhibition (88.26% and 92.6%) at 25 and 50 µg/mL concentrations. These results achieves that n-hexane crude extract taking more effective activity among the other crude extracts.


Acalypha india, HIV, MTT assay, reverse transcriptase, trypan blue.


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Article Dates:

Received: 12 June 2022; Accepted: 10 August, 2022; Published: 3 September, 2022

How To Cite:

Prasad Paindla, & Estari Mamidala. (2022). Inhibition of Human Immunodeficiency Virus (HIV-1) Reverse Transcriptase by Acalypha indica (L.) Plant Leaves Extract. The American Journal of Science and Medical Research, 8(3), 1–4. https://doi.org/10.5281/zenodo.7213389

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